COURSE CODE: PBS 507
COURSE CODE: Introduction to Crop Biotechnology
NUMBER OF UNITS: 3 Units
COURSE DURATION: Three hours per week
Course Coordinator: Dr. I. O. Daniel
Office Location: Room 245, COLPLANT
Definition of biotechnology, its branches and its relationship to basic sciences. Importance of biotechnology in crop improvement and production. Nucleic acid hybridization, function of endonuclease, polymerase and other enzymes. Role of southern blot, restriction fragment length polymorphism and other techniques in gene mapping. Transformation and production of transgenic crops. Protoplast, cell, tissue and organ culture, in-vitro pollen germination and fertilization, protoplasm and cell fusion, crop micro-propagation and somatic embryogenesis in rapid multiplication.
Practical: Preparation of plant growth media, aseptic transfer of explants, micro-propagation of plants.